bottom 96 well plate  (PromoCell)

Journal: Microorganisms

Article Title: Anti-Hair Loss Activity of Healthy Human Scalp-Derived Staphylococcus capitis KMH304 Ferment Filtrate in Human Hair-Follicle Dermal Papilla and Keratinocyte Cells

doi: 10.3390/microorganisms14040929

Figure Lengend Snippet: Effect of SCFF on HFDPC and HaCaT cell viability and proliferation. HFDPCs and HaCaT cells were treated with different concentrations of SCFF as indicated for 24 h. ( a ) HFDPC and ( b ) HaCaT viability and proliferation were assessed at various SCFF concentrations (50, 100, 250, 500, 1000, 2500, 5000 μg/mL) using a CCK-8 assay. Values are mean ± SD of three independent experiments. ( a – c ) *** p < 0.001, ** p < 0.01 and * p < 0.05 versus NC group (100%). ( c ) Representative images of cellular proliferation activity in HFDPCs and HaCaT cells after 48 h of incubation ( n = 3; Scale bar = 200 μm). SCFF, Staphylococcus capitis KMH304 ferment filtrate; HFDPCs, human follicle dermal papilla cells; NC, negative control.

Article Snippet: HFDPCs (C-12071; Promocell, Heidelberg, Germany) were cultured in Follicle Dermal Papilla Cell Growth Medium (C-26501; Promocell) supplemented with 1% antibiotic–antimycotic (Gibco; Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: CCK-8 Assay, Activity Assay, Incubation, Negative Control

Journal: Microorganisms

Article Title: Anti-Hair Loss Activity of Healthy Human Scalp-Derived Staphylococcus capitis KMH304 Ferment Filtrate in Human Hair-Follicle Dermal Papilla and Keratinocyte Cells

doi: 10.3390/microorganisms14040929

Figure Lengend Snippet: Hair growth-related and AGA-inducing signature gene expression following SCFF treatment in HFDPCs. HFDPCs were treated with different SCFF concentrations for 24 h, and gene expression was evaluated using qPCR. ( a ) Hair growth-related factors (KGF, IGF-1, and HGF). ( b ) AGA-inducing factors (AR and TGF-β2). MXD at 5 μg/mL was used as PC for TGF-β2, and 2.5 μg/mL MXD was used as PC for other markers. Data are mean ± SD of three independent experiments. *** p < 0.001, ** p < 0.01 and * p < 0.05 versus NC group (100%). SCFF, Staphylococcus capitis KMH304 ferment filtrate; HFDPCs, human follicle dermal papilla cells; MXD, minoxidil; PC, positive control.

Article Snippet: HFDPCs (C-12071; Promocell, Heidelberg, Germany) were cultured in Follicle Dermal Papilla Cell Growth Medium (C-26501; Promocell) supplemented with 1% antibiotic–antimycotic (Gibco; Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Gene Expression, Positive Control

Journal: Microorganisms

Article Title: Anti-Hair Loss Activity of Healthy Human Scalp-Derived Staphylococcus capitis KMH304 Ferment Filtrate in Human Hair-Follicle Dermal Papilla and Keratinocyte Cells

doi: 10.3390/microorganisms14040929

Figure Lengend Snippet: The regulation of cellular senescence by SCFF in HFDPCs. HFDPCs were treated with different concentrations of SCFF for 24 h, followed by analysis using qPCR and SA-β-gal staining. ( a , b ) Anti-aging factors (SIRT1, SIRT7, COL13A1, and p21). ( c ) SA-β-gal staining in young (P6) and senescent (P16) passage cells. Quantitative and representative images of SA-β-gal-positive cells compared with senescent cells (Scale bar = 200 μm). For SIRT, 1 μg/mL fisetin (Fis) was used as a positive control (PC), and for COL13 and p21, 1 μg/mL Fis was used as PC. Data are the mean ± SD of three independent experiments. **** p < 0.0001, *** p < 0.001, ** p < 0.01, and * p < 0.05 versus the negative control (NC) and the senescent group (100%). SCFF, Staphylococcus capitis KMH304 ferment filtrate; HFDPCs, human follicle dermal papilla cells.

Article Snippet: HFDPCs (C-12071; Promocell, Heidelberg, Germany) were cultured in Follicle Dermal Papilla Cell Growth Medium (C-26501; Promocell) supplemented with 1% antibiotic–antimycotic (Gibco; Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Staining, Positive Control, Negative Control

Journal: Microorganisms

Article Title: Anti-Hair Loss Activity of Healthy Human Scalp-Derived Staphylococcus capitis KMH304 Ferment Filtrate in Human Hair-Follicle Dermal Papilla and Keratinocyte Cells

doi: 10.3390/microorganisms14040929

Figure Lengend Snippet: Overall effect of SCFF in HFDPCs and HaCaT cells. In keratinocytes, SCFF enhanced barrier function and antioxidant activity. SCFF not only regulated cellular aging through longevity-, collagen-, and p21-related genes, but also promoted hair growth by increasing the expression of hair-growth–associated factors and suppressing hair loss-inducing factors in HFDPCs. SCFF, Staphylococcus capitis KMH304 ferment filtrate; HFDPCs, human follicle dermal papilla cells.

Article Snippet: HFDPCs (C-12071; Promocell, Heidelberg, Germany) were cultured in Follicle Dermal Papilla Cell Growth Medium (C-26501; Promocell) supplemented with 1% antibiotic–antimycotic (Gibco; Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Antioxidant Activity Assay, Expressing

Journal: BMC Biotechnology

Article Title: A novel cell-penetrating peptide supports hair follicle growth through anti-inflammatory and growth factor–associated mechanisms in preclinical models

doi: 10.1186/s12896-026-01130-4

Figure Lengend Snippet: Screening process to determine peptides with hair-restoring properties. ( A ) Human Follicle Dermal Papilla Cell (HFDPCs) viability of over 200 cell-penetrating peptides, with EGF as the positive control. The red dots mark the peptides with the highest cell viability. ( B ) Western Blot results showing the upregulation of β-catenin and p-ERK proteins of 22 chosen peptides compared to the control. Peptide No.218 (in red rectangle) showed the highest expression level of β-catenin and p-ERK proteins, and was chosen for subsequent analyses. ( C ) Cell-penetrating capability of DualPep-ALO was evaluated using FITC-conjugated peptides in HFDPCs. Cells were treated with FITC-DualPep-ALO or FITC-TAT (2.5 µM, 2 h), followed by extensive washing and trypsinization to remove membrane-bound peptides. Intracellular fluorescence intensity was quantified by flow cytometry. Each experiment was performed three times

Article Snippet: HFDPCs were cultured in Human Follicle Dermal Papilla Cell Growth Medium (PromoCell, Heidelberg, Germany) supplemented with fetal calf serum, bovine pituitary extract, basic fibroblast growth factor, and insulin.

Techniques: Positive Control, Western Blot, Control, Expressing, Membrane, Fluorescence, Flow Cytometry

Journal: BMC Biotechnology

Article Title: A novel cell-penetrating peptide supports hair follicle growth through anti-inflammatory and growth factor–associated mechanisms in preclinical models

doi: 10.1186/s12896-026-01130-4

Figure Lengend Snippet: Effects of DualPep-ALO on cell viability. ( A ) Mouse macrophage viability after 24-hour treatment with various concentrations of DualPep-ALO (0.64, 3.2, 16, 80 µM), compared with L-NMMA (25 µM). ( B ) Human Follicle Dermal Papilla Cells viability after treatment with DualPep-ALO (3.2, 16, 80 µM) for 24, 48, and 72 hours, compared with minoxidil (20 µM). Data are presented as mean ± SD. *p < 0.05 vs. negative control at the same time point. Each experiment was performed three times

Article Snippet: HFDPCs were cultured in Human Follicle Dermal Papilla Cell Growth Medium (PromoCell, Heidelberg, Germany) supplemented with fetal calf serum, bovine pituitary extract, basic fibroblast growth factor, and insulin.

Techniques: Negative Control

Journal: BMC Biotechnology

Article Title: A novel cell-penetrating peptide supports hair follicle growth through anti-inflammatory and growth factor–associated mechanisms in preclinical models

doi: 10.1186/s12896-026-01130-4

Figure Lengend Snippet: Antioxidant enzyme activities in Human Follicle Dermal Papilla Cells (HFPDCs) treated with DualPep-ALO. DualPep-ALO (3.2, 16, 80 µM) enhanced the activities of ( A ) Superoxide dismutase (SOD) and ( B ) catalase (CAT) in HFPDCs after oxidative stress induction with H 2 O 2 (400 µM). L-Ascorbic acid (100 µM) was used as a positive control. Data are expressed as mean ± SD. *p < 0.05 vs. negative control; #p < 0.05 vs. H 2 O 2 -treated group. Each experiment was performed three times

Article Snippet: HFDPCs were cultured in Human Follicle Dermal Papilla Cell Growth Medium (PromoCell, Heidelberg, Germany) supplemented with fetal calf serum, bovine pituitary extract, basic fibroblast growth factor, and insulin.

Techniques: Positive Control, Negative Control